Bovine viral diarrhea virus 1 (ATCC® VR-1561)

Classification: Flaviviridae, Pestivirus  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Flaviviridae, Pestivirus
Agent Bovine viral diarrhea virus 1
Strain New York 1
Common Name Bovine viral diarrhea virus 1
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Derived from same strain by passaging in the absence of bovine serum. Passage infected cells for optimal propagation of virus.
Effect on Host
No CPE, detect by immunofluorescence or molecular-based procedures
Recommended Host
BT (ATCC® CRL-1390™)
Growth Conditions

Recommendations for Infection:  Plate cells 24-48 hours prior to infection and infect when cultures are 60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation:  Incubate infected culture for 4-21 days at 37°C in a humidified 5% CO2 atmosphere.

Effect on Host
No CPE, detect by immunofluorescence or molecular-based procedures
Name of Depositor M Snyder, GA Erickson, The National Animal Disease Center
Special Collection ATCC
Source Spleen from calf with diarrhea, New York, 1948
References

Baker JA. . Am. J. Vet. Res. 15: 525, 1954.

Kim SG, Dubovi EJ. A novel one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhea virus in bulk milk, blood, and follicular fluid samples. Biologicals. 31: 103-106, 2003. PubMed: 12770539

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation