Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 700699)

Strain Designations: Mu50 [NRS1]  /  Type Strain: no  /  Biosafety Level: 2

Permits And Restrictions

View Permits

Deposited As Staphylococcus aureus Rosenbach
Strain Designations Mu50 [NRS1]
Application
Drug discovery
Isolation
Isolated from pus and debrided tissue at surgical incision in sternum of 4 month-old infant, Japan, 1996.
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype pvl negative
Genome Sequenced Strain

Staphylococcus aureus subsp. aureus Mu50 DNA, complete genome

Type Strain no
Antibiotic Resistance Methicillin resistant
Oxacillin resistant
Reduced vancomycin susceptibility
Comments
Degree of resistance tends to decrease depending on the culture condition. Recommend propagating strain in Brain Heart Infusion with 4 mg/L vancomycin (ATCC Medium 2174).
Genome sequenced strain

MRSA
SCCmec: Type II
spa type Ridom: t002
spa type Kreiswirth: TJMBMDMGMK
pvl gene amplification: Negative

Phenotypic and genotypic information for ATCC MRSA strains, such as SCCmec, PFGE, or spa typing, is provided for informational purposes only. This information was compiled from testing performed by one or more sources, including ATCC, strain depositor, published data, or contracted research.
Medium ATCC® Medium 2174: Brain heart infusion medium with vancomycin 4 mcg/ml
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype pvl negative
Name of Depositor K Hiramatsu
Special Collection NCRR Contract
Isolation
Isolated from pus and debrided tissue at surgical incision in sternum of 4 month-old infant, Japan, 1996.
References

Hiramatsu K, et al. Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin. Lancet 350: 1670-1673, 1997. PubMed: 9400512

Hiramatsu K, et al. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J. Antimicrob. Chemother. 40: 135-136, 1997. PubMed: 9249217

Keiichi Hiramatsu, personal communication

Monecke S, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6(4): e17936, 2011. PubMed: 21494333

Cross References

Nucleotide (GenBank) : BA000017 Staphylococcus aureus subsp. aureus Mu50 DNA, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation