Caov-4 (ATCC® HTB-76)

Organism: Homo sapiens, human  /  Tissue: ovary; Derived from Metastatic Site: subserosa of fallopian tube  /  Disease: adenocarcinoma

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Organism Homo sapiens, human
Tissue ovary; Derived from Metastatic Site: subserosa of fallopian tube
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 45 years
Gender female
Ethnicity Caucasian
Karyotype (P5) hypotriploid and hypertetraploid with abnormalities including breaks, dicentrics, minute chromatin bodies, fragmentations, secondary constrictions and large subtelocentric and submetacentric markers
Images
Clinical Data
45 years
Caucasian
female
Complete Growth Medium The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes).Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: complete growth medium supplemented with an additional 40% fetal bovine serum and 10% (v/v) DMSO
Culture Conditions
Temperature: 37°C
Atmosphere: air, 100%
Isoenzymes
AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 2
Name of Depositor J Fogh
References

Yaginuma Y, et al. Analysis of the Rb gene and cyclin-dependent kinase 4 inhibitor genes (p16INK4 and p15INK4B) in human ovarian carcinoma cell lines. Exp. Cell Res. 233: 233-239, 1997. PubMed: 9194486

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
Restrictions

The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the cells subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with the Office of Technology Development, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10065. Contact Tingting Zhang-Kharas, Direct Phone: 646-888-1083, Reception: 646-888-1080, Email: zhangkht@mskcc.org

References

Yaginuma Y, et al. Analysis of the Rb gene and cyclin-dependent kinase 4 inhibitor genes (p16INK4 and p15INK4B) in human ovarian carcinoma cell lines. Exp. Cell Res. 233: 233-239, 1997. PubMed: 9194486