pUR19 (ATCC® 77298)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: NC Barbet, AM Carr

Designations pUR19
Depositors NC Barbet, AM Carr
Biosafety Level 1
Vector Information
Size (kb): 5.5000000000000000
Vector: pUR19 (plasmid)
Promoters: Promoter lac
Construction: pUC19, pON163
Marker(s):ampR,ura4+
Construct size (kb): 5.5
Features: insert detection: lacZ'
marker(s): ampR
marker(s): ura4+
promoter: lac
replicon: ars1
replicon: pMB1
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.5; BamHI--5.5; HindIII--5.5; PstI--5.5.
Shuttle vector permitting visual detection of recombinants by beta-galactosidase alpha complementation.
Constructed by inserting a 1.7 kb ClaI fragment containing ura4 and a 1.2 kb ClaI fragment containing ars1 into the NdeI site modified with a ClaI linker.
The order of the major features in this plasmid is: ura4 - ars1 - EcoRI/MCS/HindIII/lacZ' - pMB1 ori - ampR.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Barbet N, et al. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe. Gene 114: 59-66, 1992. PubMed: 1587485

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component of:ATCC 87686
Shipped frozen
Shipping Information Freeze dried E. coli containing the plasmid
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