Parainfluenza 3 (ATCC® VR-1782)

Classification: Paramyxoviridae, Paramyxovirus  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Paramyxovirus
Agent Parainfluenza 3
Strain ATCC-2011-5
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions  -70°C or colder
Effect on Host
CPE, refractile cell rounding in culture
CPE, refractile cell rounding
Recommended Host

Production Host:  LLC-MK2 Derivative (ATCC® CCL-7.1)

LLC-MK2 Derivative (ATCC CCL-7.1)
Growth Conditions
Temperature: 35°C
Recommendations for Infection: Plate cells 24-48 hours in advance and infect when cultures are 80-90% confluent. Remove medium, rinse with plain EMEM, and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 9-14 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, refractile cell rounding in culture
CPE, refractile cell rounding
Name of Depositor ATCC
Source
Clinical sample from a patient positive for PIV 3 in Virginia, June 2011
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation