6-23 (Clone 6) (ATCC® CRL-1607)

Organism: Rattus norvegicus, rat  /  Cell Type: C cell  /  Tissue: thyroid  /  Disease: medullary thyroid carcinoma

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Organism Rattus norvegicus, rat
Tissue thyroid
Cell Type C cell
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease medullary thyroid carcinoma
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
neurotensin; calcitonin
Comments
Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 mL of 0.25% trypsin and let the culture sit at 37°C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor R Gagel, FN Zeytinoglu
References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046