SUP-B15 (ATCC® CRL-1929)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: bone marrow  /  Disease: acute lymphoblastic leukemia

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Organism Homo sapiens, human
Tissue bone marrow
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute lymphoblastic leukemia
Age 8 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor temperature
Karyotype 46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present.
Derivation
This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL.
Clinical Data
8 years
Caucasian
male
­
Antigen Expression
CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR +
Genes Expressed
immunoglobulin (cytoplasmic)
Cellular Products
immunoglobulin (cytoplasmic)
Comments
The cells express multiple B lineage markers, but do not express T cell markers.

The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens.

They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus.


Complete Growth Medium Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 105 viable cells/mL and maintain between 5 X 105 and 2 X 106 cells/mL.
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,14
D16S539: 11,12
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 15,17
Population Doubling Time 18 hrs
Name of Depositor SD Smith
References

Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022

Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516

Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827

Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755

Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022

Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516

Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827

Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755

Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197