Bovine herpesvirus 1 (ATCC® VR-188)

Classification: Herpesviridae, Varicellovirus  /  Product Format: frozen 1 mL per vial

Classification Herpesviridae, Varicellovirus
Deposited As Infectious bovine rhinotracheitis
Agent Bovine herpesvirus 1
Strain Los Angeles
Common Name Infectious bovine rhinotracheitis virus, Bovine rhinotracheitis virus
Applications
Produces hemolysin but not HA. Stable at -60°C. Single antigenic group.
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-188 uninfected and infected images
Comments Produces hemolysin but not hemagluttinin.
Effect on Host CPE, rounding and sloughing
Recommended Host EBTr (NBL-4) (ATCC® CCL-44™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3 days
Effect on Host CPE, rounding and sloughing
Name of Depositor DG McKercher, UC Davis School of Veterinary Medicine
Source Dairy cattle, Los Angeles County, California, 1956
References

Madin SH, et al. Isolation of the infectious bovine rhinotracheitis virus. Science 124: 721-722, 1956. PubMed: 13371311