Kluyveromyces lactis (Dombrowski) van der Walt, teleomorph (ATCC® 8585)

Strain Designations: [61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174]  /  Product Format: freeze-dried

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Strain Designations [61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174]
Application
degrades whey permeate
hydrolyzes whey lactose
produces L-methionine
produces beta-galactosidase beta-D-galactosidase
produces killer toxin
produces L-methionine from whey permeate
Biosafety Level 1
Product Format freeze-dried
Type Strain no
Genotype a [k1+ k2+]
Preceptrol® no
Genome Sequenced Strain

Yes

Mating Type mates with ATCC 8651
Comments
regulation of pyruvate dehydrogenase activity by E1alpha subunit levels
resistant to killer toxin produced by Schwanniomyces occidentalis ATCC 44252
carries two linear DNA plasmids, pGK11 and pGK12
transcriptional regulation of beta-galactosidase gene
effect of lactose permeabilization on beta-galactosidase production
Nonflocculating
killer, immune
Genome sequencing strain (Genolevures Consortium, France).
Sequenced mitochondrial genome.
Medium Medium 200: YM agar or YM broth
Growth Conditions
Temperature: 24.0°C
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor Lederle Labs.
Chain of Custody
ATCC <<--Lederle Labs.<<--N.C. Laffer 61, type C (Zygosaccharomyces lactis)
Isolation
creamery, USA
Cross References

Nucleotide (GenBank) : X72499 nucleotide sequence of KlGGS1 gene

Nucleotide (GenBank) : X81421 K.lactis gene for alpha-alpha-trehalose 1-D-glucohydrolase.

Nucleotide (GenBank) : CR382121 Kluyveromyces lactis strain NRRL Y-1140 chromosome A complete sequence.

References

Gunge N, et al. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character. J. Bacteriol. 145: 382-390, 1981. PubMed: 6257636

Wickerham LJ, Burton KA. Occurrence of yeast mating types in nature1,3. J. Bacteriol. 63: 449-451, 1952. PubMed: 14938317

Lacy LR, Dickson RC. Transcriptional regulation of the Kluyveromyces lactis beta- galactosidase gene. Mol. Cell. Biol. 1: 629-634, 1981. PubMed: 9279376

. . Biotechnol. Tech. 6: 289-292, 1992.

Schaffrath R, Meacock PA. Kluyveromyces lactis killer plasmid pGKL2: molecular analysis of an essential gene, ORF5. Yeast 11: 615-628, 1995. PubMed: 7483835

Bellal M, et al. Structural differences in parietal peptidomannans from flocculating strains of Kluyveromyces lactis. Can. J. Microbiol. 41: 323-335, 1995.

Luyten K, et al. Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake. Eur. J. Biochem. 217: 701-713, 1993. PubMed: 8223613

Dickson RC, Markin JS. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis. J. Bacteriol. 142: 777-785, 1980. PubMed: 6769910

Siso MI. Beta-galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem. 29: 565-568, 1994.

Bellal M, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 30: 641-648, 1995.

. . Enzyme Microb. Technol. 16: 303-310, 1994.

Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863

Zeeman AM, et al. Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 16: 611-620, 2000. PubMed: 10806423

Chen WB, et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000. PubMed: 11097913

Kitamoto HK, Nakahara T. Isolation of an L-methionine-enriched mutant of Kluyveromyces lactis grown on whey permeate. Process Biochem. 29: 127-131, 1994.

Dujon B, et al. Genome evolution in yeasts. Nature 430: 35-44, 2004.

Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000.

Bolotin-Fukuhara M, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis. FEBS Lett. 487: 66-70, 2000.

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009.

Zivanovic Y, et al. Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces lactis. FEMS Yeast Res. 5: 315-322, 2005. PubMed: 15691736

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation