DDT1-MF-2 (ATCC® CRL-12051)

Organism: Mesocricetus auratus, hamster, Syrian golden  /  Disease: leiomyosarcoma

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Organism Mesocricetus auratus, hamster, Syrian golden
Product Format frozen
Culture Properties adherent
Biosafety Level 1
Disease leiomyosarcoma
Gender male
Clinical Data
male
Receptor Expression
androgen receptor, positive; glucocorticoid; alpha 1 adrenergic; beta 2 adrenergic
Comments
Growth of DDT1-MF-2 cells is stimulated by androgens and inhibited by glucocorticoids.
Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene.
Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase.
This a deposit of DDT1-MF-2 (see ATCC CRL-1701) for patent purposes.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week
To dislodge adherent cells, remove all but 5 to 10 ml of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Name of Depositor Merck & Co., Inc.
References

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513