DNA from Chlamydophila pneumoniae strain CM-1 (ATCC® VR-1360D)

Organism: Chlamydophila pneumoniae  / 

Product Format frozen
Total DNA from infected cells, ≥1 µg/mL in 1X TE buffer
100 µL per vial, shipped frozen
Storage Conditions -70°C or colder
Applications
DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Comments
Chlamydophila DNA, in total DNA isolated from HEp-2 cells (ATCC CCL-23) infected with Chlamydophila pneumoniae strain CM-1 (ATCC VR-1360). This product was prepared using methods known to inactivate the infecting agent. DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications
  1. Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
  2. Concentration is verified as ≥1 µg/mL DNA by Picogreen® assay.
  3. Purity is confirmed by OD260/OD280 ratio (≥1.7) of bulk DNA before dilution for product filling.
  4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µl of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.
  5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
  6. Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.