DNA from Chlamydophila pneumoniae strain CM-1 (ATCC® VR-1360D™)

Organism: Chlamydophila pneumoniae  / 

Product Format frozen

PRODUCT INFORMATION:

Total DNA from infected cells, >1 µg/mL in 1X TE buffer

100 µL per vial, shipped frozen

Storage Conditions -70°C or colder
Applications
DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Comments
Chlamydophila DNA, in total DNA isolated from HEp-2 cells (ATCC CCL-23) infected with Chlamydophila pneumoniae strain CM-1 (ATCC VR-1360). This product was prepared using methods known to inactivate the infecting agent. DNA isolated from infected cells is appropriate for PCR and other molecular biology applications.
Biosafety Level 1
Quality Control Specifications
1. Store at -20°C or colder. -70°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).

2. Concentration is verified as >1 µg/mL DNA by Picogreen® assay.

3. Purity is confirmed by OD260/OD280 ratio (>1.7) of bulk DNA before dilution for product filling.

4. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis of 20 µl of product on a 0.8% agarose gel, visualized by Ethidium bromide staining.

5. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.

6. Identity is confirmed by sequencing of approximately 300 bp to 1Kb of the PCR amplicon.