CHLA-03-AA (ATCC® CRL-3035)

Organism: Homo sapiens, human  /  Tissue: Brain (Temporal lobe)  /  Disease: anaplastic astrocytoma

Organism Homo sapiens, human
Tissue Brain (Temporal lobe)
Morphology rounded-floating or loosely attached (neuronal-like)
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease anaplastic astrocytoma
Age 9 years
Gender Female
Storage Conditions liquid nitrogen vapor phase
Images CRL-3035 Micrograph
Derivation CHLA-03-AA was established from a 9 year old girl with recurrent anaplastic astrocytoma (AA). Cells were isolated from freshly resected brain tumor and were cultured in neurobasal medium after mechanical disruption.
Comments

Cultures grow as floating aggregates of round cell clusters and as lightly attached neuronal-like cells.

This cell line has been continuously carried in culture for over 18 months, and in neurobasal medium grows as spheres of varying sizes.

The generation of this cell line was made possible with the support of the Michael Hoefflin Foundation for Children's Cancer to Children’s Hospital Los Angeles, with the goal of making pediatric brain tumor lines available to the research community.

Complete Growth Medium DMEM:F12 Medium (ATCC 30-2006) with 20 ng/mL human recombinant EGF, 20 ng/mL human recombinant basic FGF, and B-27 Supplement (Invitrogen, Cat. No.17504) to a final concentration of 2% (v/v)
Subculturing Cultures can be maintained by the addition of fresh medium when there are numerous, healthy-appearing clusters present in suspension and pH of medium has decreased. Alternatively, subculture by gently dislodging the cells in the spent culture medium by pipetting across the monolayer or by rapping the side of the flask sharply with the palm of your hand (the latter is only preferable when working with larger flasks), followed by centrifugation with subsequent resuspension in ¼ volume of the conditioned medium and ¾ volume fresh medium.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended.
Medium renewal: Add fresh medium twice weekly (depending on cell density).
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
D5S818: 11
D13S317: 10, 11
D7S820: 9, 10
D16S539: 11, 12
vWA: 16, 18
TH01: 6, 9
Amelogenin: X
TPOX: 8, 9
CSF1PO: 10
Name of Depositor A Erdreich-Epstein, Saban Research Institute, Children’s Hospital Los Angeles