S1A(Thy-1-b) (ATCC® TIB-231)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

Permits and Restrictions

View Permits

Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease lymphoma
Strain BALB/c
Applications
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derivation
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
Genes Expressed
The cells secrete a mutant Thy-1 glycoprotein.
Cellular Products
The cells secrete a mutant Thy-1 glycoprotein.
Comments
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
References

Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421