S1A(Thy-1-b) (ATCC® TIB-231)

Organism: Mus musculus, mouse  /  Cell Type: T lymphocyte  /  Disease: lymphoma

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Organism Mus musculus, mouse
Cell Type T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphoma
Strain BALB/c
Applications
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Derivation
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
Genes Expressed
The cells secrete a mutant Thy-1 glycoprotein.
Cellular Products
The cells secrete a mutant Thy-1 glycoprotein.
Comments
This line is a Class B Thy-1 negative (Thy-1-) mutant derived from S1A.TB.4.8.2 (ATCC TIB-27).
The mutation affects the biosynthesis and/or addition of the glycophospholipid anchor.
The line may be useful in studies of the synthesis and assembly of glycophospholipid linked membrane proteins.
The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine.
Complete Growth Medium Dulbecco's modified Eagle's medium, 90%; heat-inactivated horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor R Hyman
References

Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Conzelmann A, et al. No glycolipid anchors are added to Thy-1 glycoprotein in Thy-1-negative mutant thymoma cells of four different complementation classes. Mol. Cell. Biol. 8: 674-678, 1988. PubMed: 2895421