JAWSII (ATCC® CRL-11904)

Organism: Mus musculus, mouse  /  Cell Type: immature dendritic cell; monocyte  /  Tissue: bone marrow  / 

Permits and Restrictions

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Organism Mus musculus, mouse
Tissue
bone marrow
Cell Type immature dendritic cell; monocyte
Product Format frozen
Morphology monocyte
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Strain C57BL/6
Storage Conditions liquid nitrogen vapor phase
Complete Growth Medium Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
Subculturing
Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Name of Depositor ZymoGenetics, Inc.
References

MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997

Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997

Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998