Mumps virus (ATCC® VR-106)

Classification: Paramyxoviridae, Rubulavirus  /  Product Format: frozen 1 mL per vial

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Classification Paramyxoviridae, Rubulavirus
Agent Mumps virus
Strain Enders
Applications
This strain was used for preparation of skin test and viral V and S complement fixation antigens.
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments This strain was used for preparation of skin test and viral V and S CF antigens. It is of low virulence for human volunteers. GenBank: GU980052.1
Effect on Host
CPE, cell rounding, syncytia formation, and sloughing
Recommended Host
SL-29 (ATCC® CRL-1590™)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 36°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 5-11 days
Effect on Host
CPE, cell rounding, syncytia formation, and sloughing
Name of Depositor JF Enders, Children's Medical Center, Boston, Massachusetts
Source Pooled saliva from patients, Massachusetts
References

Andrews CH. A short description of the Myxovirus group (Influenza and related viruses). Virology. 2: 176-184, 1955. PubMed: 13267985.

Enders JF, et al. Immunity in mumps: I. Experiments with monkeys (Macacus mulatta). The development of complement-fixing antibody following infection and experiments on immunization by means of inactivated virus and convalescent human serum. J. Exp. Med. 81: 93-117, 1945. PubMed: 19871448.

Henle G, et al. The reactivity of various human sera with mumps complement fixation antigens. J. Exp. Med. 1: 133-147, 1948. PubMed: 18871883.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation