SH-34 (ATCC® CRL-2405)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
This monoclonal antibody can be used for the study of murine and rat hematopoietic and lymphopoietic cell lineages.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the glycolipid asialo GM1 adsorbed to naked Salmonella. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against asialo-GM1
Cellular Products
immunoglobulin; monoclonal antibody; against asialo-GM1
Comments The antibody is specific for asialo-GM1. This antibody recognizes and kills by complement lysis cells known to express asialo GM1. 

Most natural killer (NK) cells and cytotoxic T cells (CTL), as well as a proportion of other myeloid and lymphoid cells express asialo GM1. 

The SH-34 antibody specifically lyses asialo GM1-expressing macrophages in the presence of complement and removes NK cells in vitro from spleen cell populations. RefSolomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206 

Complete Growth Medium Modified Dulbecco's medium, 95%; fetal bovine serum, 5%
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density).
Cryopreservation
Culture medium, 85%; fetal bovine serum, 10%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Isotype IgM
Name of Depositor TJ Higgins
References

Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562

Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206

Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802

Basic Documentation
References

Stout RD, et al. Evidence that expression of asialo-GM1 may be associated with cell activation. Correlation of asialo-GM1 expression with increased total cellular RNA and protein content in normal thymocyte and spleen cell populations. J. Immunol. 139: 2123-2129, 1987. PubMed: 2443562

Solomon FR, Higgins TJ. A monoclonal antibody with reactivity to asialo GM1 and murine natural killer cells. Mol. Immunol. 24: 57-65, 1987. PubMed: 3614206

Suttles J, et al. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes. J. Immunol. 136: 1586-1591, 1986. PubMed: 2936802