Adeno-associated virus 4 (ATCC® VR-646)

Classification: Parvoviridae, Dependovirus, Adeno-associated virus-4  /  Product Format: frozen

Classification Parvoviridae, Dependovirus, Adeno-associated virus-4
Agent Adeno-associated virus 4
Strain none
Positive for Mycoplasma Yes
Biosafety Level 2
Isolation
Monkey cells infected with SV-15 virus
Product Format frozen
Storage Conditions -70°C or colder
Comments
Depends upon adenovirus for replication. *Helper virus may be inactivated at 56C for 30 min.
Effect on Host
Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
AAV produces no CPE.  Helper adenovirus induces refractile rounding and cell sloughing. 
Non-cytopathic without helper virus
Recommended Host
monkey kidney cells; suckling mouse

Recommended Host:  LLC-MK2 (ATCC® CCL-7.1TM)

Growth Conditions
Temperature: 37.0°C
Duration: Evidence of virus growth can be expected within 2-3 days. For best results cells should be 24-48 hours old and 80%-90% confluent (not 100%) at the time of infection.
Effect on Host
Cytopathic effect: AAV produces no CPE. Helper adenovirus induces refractile rounding and cell sloughing.
AAV produces no CPE.  Helper adenovirus induces refractile rounding and cell sloughing. 
Non-cytopathic without helper virus
Name of Depositor WP Parks
Source
Monkey cells infected with SV-15 virus
References

Chiorini JA, et al. Cloning of adeno-associated virus type 4 (AAV4) and generation of recombinant AAV4 particles. J. Virol. 71: 6823-6833, 1997. PubMed: 9261407

Parks WP, et al. Physical assay and growth cycle studies of a defective adeno-satellite. J. Virol. 1: 171-180, 1967. PubMed: 4990036

Cross References

Nucleotide (GenBank) : U89790 Adeno-associated virus 4, complete genome.

Nucleotide (GenBank) : NC_001829 Adeno-associated virus 4, complete genome.