Betacoronavirus 1 (ATCC® VR-1558)

Classification: Coronaviridae, Betacoronavirus  /  Product Format: frozen 1 mL per vial

Classification Coronaviridae, Betacoronavirus
Deposited As Human coronavirus OC43
Agent Betacoronavirus 1
Strain OC43
Common Name Human coronavirus OC43
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Image of Host Effects for VR-1558
Comments VR-1558™ is a TC-adapted item derived from virus passaged in mice (ATCC® VR-759™).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™).
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Recommended Host
HCT-8 (ATCC® CCL-244™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Name of Depositor JO Fleming, University of Wisconsin, Madison
Special Collection ATCC
Chain of Custody ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Source Man with cold-like illness. Treated to remove mycoplasma at ATCC.
References

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356

Cross References

Nucleotide (GenBank) : NC_005147.1 Human coronavirus OC43, complete genome