SML, clone 4D8 (ATCC® CRL-2311)

Organism: Saimiri boliviensis boliviensis, monkey, bolivian squirrel  /  Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transforme  / 

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Organism Saimiri boliviensis boliviensis, monkey, bolivian squirrel
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties The cells grow in loose clumps., suspension
Biosafety Level 2
Gender male
Applications
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Karyotype The SML, clone 4D8 cells exhibit a karyotype consistent with the subspecies Saimiri boliviensis boliviensis; the chromosomal count was 44 with an XY sex chromosome constitution., G-banding revealed a pattern of the Bolivian karyomorph for the subspecies Saimiri boliviensis boliviensis with a submetacentric chromosomal pair 15 and an acrocentric chromosomal pair 16 resulting in six pairs of autosomal acrocentric chromosomes., The karyotype was heterozygous for the C-band polymorphism in the short arm of chromosome 14 and lacked the chromosome 5 C-band positive polymorphism found in Guyanese animals, Saimiri sciureus sciureus.
Derivation
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
Clinical Data
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
male
Antigen Expression
CD20 +
Genes Expressed
CD20 +
Comments
SML, clone 4D8 is a B lymphocyte cell line established in 1995 by transformation of mononuclear cells from a male squirrel monkey with Epstein-Barr virus derived from B95-8 cells (ATCC CRL-1612).
The resulting cells were cloned by limiting dilution.
CITES Permit
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 x 10(5) viable cells/ml.
Maintain cultures at cell concentrations between 5 x 10(5) and 2 x 10(6) viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor JG Scammell
Year of Origin 1995
References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Reynolds PD, et al. Cloning and expression of the glucocorticoid receptor from the squirrel monkey (Saimiri boliviensis boliviensis), a glucocorticoid-resistant primate. J. Clin. Endocrinol. Metab. 82: 465-472, 1997. PubMed: 9024238