Chlamydophila pecorum (Fukushi and Hirai) Everett et al. (ATCC® VR-1575)

Classification: Chlamydiaceae  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

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Classification Chlamydiaceae
Agent Chlamydophila pecorum (Fukushi and Hirai) Everett et al.
Strain Ovine Polyarthritis strain IPA (Iowa polyarthritis)
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

Effect on Host
Subtle CPE; monolayer dirty looking with cell degeneration.
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 48 to 72 hours, a 5% CO2 in air atmosphere is recommended.
Effect on Host
Subtle CPE; monolayer dirty looking with cell degeneration.
Name of Depositor LA Page, ATCC
Source Derived from existing strain (This is a tissue culture adapted version of ATCC® VR-629™. ATCC® VR-629™ was isolated from sheep's joint fluid in Iowa.)
References

Page LA, Cutlip RC. Chlamydial polyarthritis in Iowa lambs. Iowa Vet. 39: 10-18, 1968.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
Basic Documentation