S1E4 [1E4] (ATCC® HB-8332™) Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) Cell Type hybridoma: B lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Strain Strain: BALB/c (B cell); BALB/c (myeloma) Derivation Spleen cells were fused with BALB/c myeloma cells. Genes Expressed immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites Comments Animals were immunized with E. tenella sporozoites.Spleen cells were fused with BALB/c myeloma cells. Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Cryopreservation culture medium 95%; DMSO, 5% Culture Conditions Temperature: 37.0°C Isotype IgG1 Name of Depositor American Cyanamid Co. References Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987