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LYK-1 (ATCC^® HB-306^™)

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) / Cell Type: hybridoma: B lymphocyte

LYK-1 ATCC^® HB-306^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Strain	Strain: S344NCR (B cell); BALB/c (myeloma)
Applications	Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.

Derivation	Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against a determinant on murine CD44 isoforms expressing variable exon V10.
Comments	Animals were immunized with a fusion protein corresponding to the products of CD44 variable exons V5 through V10. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody is specific for a determinant on murine CD44 isoforms expressing variable exon V10.

Complete Growth Medium	RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with: 0.05 mM 2-mercaptoethanol 1% Origen Hybridoma Cloning Factor 10% fetal bovine serum
Subculturing	Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 8 X 10(5) viable cells/ml.
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C

Isotype	IgG1

Name of Depositor	Y Yamashita, KS Hathcock
References	Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
References	Yamashita Y, et al. Tissue-specific and growth-regulated expression of CD44 variable exon determinants in the mouse. Cell. Immunol. 176: 22-33, 1997. PubMed: 9070314