pORF1 (ATCC® 39147)

Applications: expression vectorvector with sequences for protein export  /  Depositors: NCI-Frederick Cancer Research and Development Center

Designations pORF1
Depositors NCI-Frederick Cancer Research and Development Center
Biosafety Level 1
Host
Distribution host: Escherichia coli MH3000; MH 3000
Vector Information
Size (kb): 6.7399997711181640
Vector: pORF1 (plasmid)
Promoters: Promoter ompF
Construction: pMLB1034, pMLB841
Marker(s):ampR
Construct size (kb): 6.739999771118164
Features: marker(s): ampR
promoter: ompF
replicon: pMB1
terminator: none
enhancer: none
Applications
expression vector
vector with sequences for protein export
Comments
Requires as host MH 3000 (ATCC 35468) for viability and TK 1046 (ATCC 35467) for protein expression.
An expression vector for cytoplasmic export of a cloned gene product by formation of a fusion protein with the ompF signal sequence.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Weinstock GM, et al. Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase. Proc. Natl. Acad. Sci. USA 80: 4432-4436, 1983. PubMed: 6308625

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet