pGW10 (ATCC® 40167)

Applications: expression vector  /  Depositors: New England Biolabs, Inc., GG Wilson, New England Biolabs, Inc.

Designations pGW10
Depositors New England Biolabs, Inc., GG Wilson, New England Biolabs, Inc.
Biosafety Level 1
Vector Information
Size (kb): 11.5609998703002900
Vector: pGW10 (plasmid)
Promoters: Promoter lambda PL
Construction: pBR322, bacteriophage lambda
Marker(s):ampR,tetR
Construct size (kb): 11.56099987030029
Features: marker(s): ampR, tetR
promoter: lambda PL, lambda PR
replicon: pMB1
repressor gene: cI857
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--5.2, 4.8, 0.85, 0.5; BamHI--6.8, 4.3; EcoRI--6.8, 4.3; PvuII--11.6.
The insert contains the following restriction sites (approximate kb from the 5' HindIII site): BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9; EcoRI--8.0.
The plasmid contains the following restriction sites (starting at the EcoRI site proceeding through the pBR322 sequences): HindIII--29, 5192, 9997, 10561, 10686; BamHI--375, 7601; SalI--651; PvuII--2066; PvuI--3735, 8889; EcoRI--1, 4361.
Expression vector encoding cI857.
Distributed in aliquots of 2 ug (40 ng/ul).
The 4.3 kb BamHI fragment contains the PL and PR promoters, the cI857 gene, and 375 bp of pBR322 sequence.
The order of the major features in this plasmid is: EcoRI site - tetR - pMB1 ori - bla - bacteriophage lambda sequences Q, N, PR, rex, cI857, PR, Cr, O.
References

Chen S, Wilson GG. Method for producing the AccI restriction endonuclease and methylase. US Patent 5,004,691 dated Apr 2 1991

Geoffrey G Wilson, personal communication

Shipped frozen
Product Sheet
Product Sheet
Product Sheet