F32 VIII C4 (ATCC® CRL-1653)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension, suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain BALB/c
Applications
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Mice were immunized with purified hog renin.
Spleen cells were fused with Sp2/0 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against hog renin,H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against hog renin
Comments
Mice were immunized with purified hog renin.
Spleen cells were fused with Sp2/0 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Subculturing
Protocol: Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Medium Renewal: 2 to 3 times per week
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Isotype IgG1
Name of Depositor LT Skeggs
References

Dorer FE, et al. Purification of hog kidney remin with immobilized monoclonal antirenin. Hypertension 6: 374-382, 1984. PubMed: 6376344

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Dorer FE, et al. Purification of hog kidney remin with immobilized monoclonal antirenin. Hypertension 6: 374-382, 1984. PubMed: 6376344