BW5147.G.1.4.OUAR.1 (ATCC® CRL-1588™) Organism: Mus musculus, mouse / Cell Type: T lymphocyte General Information Characteristics Culture Method History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Organism Mus musculus, mouse Cell Type T lymphocyte Product Format frozen Morphology lymphoblast Culture Properties suspension Biosafety Level 1 Disease lymphoma Strain AKR/J Applications They can be used to create T cell hybridomas.Tested and found negative for ectromelia virus (mousepox). Antigen Expression H-2k Genes Expressed H-2k Comments The cells are ouabain resistant.They can be used to create T cell hybridomas.Tested and found negative for ectromelia virus (mousepox). Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10% Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 3 X 10 exp4 viable cells/ml. Subculture every 3 to 4 days. Name of Depositor R Hyman References Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS References Hyman R, Stallings V. Complementation patterns of Thy-1 variants and evidence that antigen loss variants "pre-exist" in the parental population. J. Natl. Cancer Inst. 52: 429-436, 1974. PubMed: 4544550