MC/CAR-Z2 [derivative of MC/CAR (ATCC CRL-8083)] (ATCC® CRL-8147™)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte

Organism Homo sapiens, human
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Age 81 years
Gender male
Ethnicity Caucasian
Applications
Resistant to 8-azaguanine.
Clinical Data
male
Caucasian
81 years
Comments
Resistant to 8-azaguanine.
Will die in HAT medium within 15 to 20 days.
Complete Growth Medium RPMI 1640 medium with 0.02 mg/ml 8-azaguanine, 80%; fetal bovine serum, 20%
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 4 X 10 exp5 and 5 X 10 exp6 cells/ml.
Population Doubling Time 20 to 24 hrs