pRS414 (ATCC® 87519)

Applications: YC-type (centromeric) shuttle vector  /  Depositors: P Hieter

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Designations pRS414
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 4.788
Vector: pRS414 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pBluescriptII KS+
Marker(s):TRP1,ampR
Construct size (kb): 4.788
Features: insert detection: lacZ'
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--4.35, 0.45; BglI--3.1, 1.7; HindIII--3.85, 0.95.
A YC-type shuttle vector with TRP1 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

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Component of: ATCC 87532
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Shipping Information Frozen phagemid in E. coli
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