pLGdelta312-polylinker (ATCC® 87522)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman

Permits and Restrictions

View Permits

Designations pLGdelta312-polylinker
Depositors DJ Stillman
Biosafety Level 1
Vector Information
Size (kb): 10.1999998092651400
Vector: pLGdelta312-polylinker (plasmid)
Promoters: Promoter CYC1 promoter (including CYC1 UAS)
Construction: pBR322
Marker(s):URA3,ampR
Construct size (kb): 10.19999980926514
Features: marker(s): URA3
marker(s): ampR
promoter: CYC1 promoter (including CYC1 UAS)
replicon: 2 micron ori
replicon: pMB1
coding sequence: lacZ
Applications
YE-type (episomal) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): XhoI--10.6; HindIII--8.0, 2.25; BglII--10.6.
This vector is one of three lacZ reporter plasmids that contain UAS (upstream activation site) elements (ATCC 87511, 87512 & 87522). They differ by polylinker sites.
DNA fragments can be inserted into these plasmids to screen for DNA elements that reduce promoter activity.
Growth Conditions
Temperature: 37.0°C
Related Products
component of:ATCC 87540
Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet