S3D3 [3D3b] (ATCC® HB-8331)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites
Cellular Products
immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites
Comments
Animals were immunized with E. tenella sporozoites.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype IgG2a
Name of Depositor American Cyanamid Co.
References

Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987

Basic Documentation
References

Wong RB, et al. Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987