pCFM526 (ATCC® 39932)

Applications: expression vector  /  Depositors: Amgen, Inc., F Lin, Amgen, Inc.

Permits and Restrictions

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Designations pCFM526
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Amgen, Inc., F Lin, Amgen, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli AM7/pMW1
Vector Information
Size (kb): 4.6500000953674320
Vector: pCFM526 (plasmid)
Promoters: Promoter lambda PL
Construction: pCFM414
Marker(s):ampR,kanR
Construct size (kb): 4.650000095367432
Features: marker(s): ampR, kanR
promoter: lambda PL
replicon: R1
terminator: lambda Toop
Applications
expression vector
Comments
Temperature sensitive. Grow at 28C.
Ampicillin is the selection marker for pCFM526, kanamycin for pMW1.
This is an expression plasmid containing a lambdaPL promoter, a ribosome binding site, a multiple cloning sequence, and a lambda Toop terminator.
This vector contains the copA region from NR1, the copB region from Beu1, and the repA and ori sequences of NR1. There is a G/C to A/T mutation at bp 598 in the copB coding region.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28.0°C
References

Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987

Morris CF. DNA plasmids. US Patent 4,710,473 dated Dec 1 1987

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet