pCYM2 (ATCC® 37824)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: JH Camonis

Designations pCYM2
Depositors JH Camonis
Biosafety Level 1
Vector Information
Size (kb): 5.4619998931884770
Vector: pCYM2 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM0 (ATCC 37820), YRp7
Marker(s):TRP1,ampR
Construct size (kb): 5.461999893188477
Features: marker(s): ampR, TRP1
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; BglII/EcoRI--3.2, 2.0; EcoRI--5.2; BamHI/PvuII--4.5, 0.51.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by deleting the HpaI (nt 1686)/BglII (nt 3463) site from pCYM0 and inserting the 879 bp BglII/EcoRI fragment containing TRP1 from YRp7.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet