pCYM2 (ATCC® 37824)

Applications: YE-type (episomal) shuttle vectorexpression vectorshuttle vector  /  Depositors: JH Camonis

Permits and Restrictions

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Designations pCYM2
Depositors JH Camonis
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.4619998931884770
Vector: pCYM2 (plasmid)
Promoters: Promoter SV40 early
Construction: pCYM0 (ATCC 37820), YRp7
Marker(s):TRP1,ampR
Construct size (kb): 5.461999893188477
Features: marker(s): ampR, TRP1
promoter: SV40 early
replicon: pMB1, 2 micron
terminator: SV40
Applications
YE-type (episomal) shuttle vector
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; BglII/EcoRI--3.2, 2.0; EcoRI--5.2; BamHI/PvuII--4.5, 0.51.
Shuttle expression vector.
Permits expression in both S. cerevisiae and mouse cells.
The EcoRI, SmaI, and BamHI sites are 3' to the unique available in-phase initiation codon under control of the SV40 early promoter.
Constructed by deleting the HpaI (nt 1686)/BglII (nt 3463) site from pCYM0 and inserting the 879 bp BglII/EcoRI fragment containing TRP1 from YRp7.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Camonis JH, et al. Of mice and yeast: versatile vectors which permit gene expression in both budding yeast and higher eukaryotic cells. Gene 86: 263-268, 1990. PubMed: 2108907

Shipped freeze-dried
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