Porcine reproductive and respiratory syndrome virus (VR-2430)

Classification: Arteriviridae, Arterivirus  /  Product Format: frozen

Classification Arteriviridae, Arterivirus
Deposited As Porcine respiratory and reproductive syndrome virus
Agent Porcine reproductive and respiratory syndrome virus
Strain ISU-55-SAH (plaque purified)
Applications
This virus produces respiratory and reproductive disease in pigs.
Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome.
Biosafety Level 2
Isolation
pneumonic pig lungs, United States
Product Format frozen
Storage Conditions -70°C or colder (or colder)
Comments
This material is bacterially contaminated. This virus produces respiratory and reproductive disease in pigs.
Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome.
Inoculate 2-3 day-old confluent monolayers with undiluted or 1:10 dilution of virus stock. After 1 hour add culture medium containing 2% FBS.
Effect on Host
Yes, in vitro effects: Cytopathic effects (degeneration, rounding, and clumping of cells)
CPE characterized by rounding of cells, clumping, clustering and degeneration of cells.
Growth Medium Dulbecco's modified Eagle's medium with 5% fetal bovine serum
Recommended Host

Recommended Host:  PSP-36-SAH cells (primate kidney)

Growth Conditions
Temperature: 37.0°C
Duration: 2-3 days at 37C in PSP-36-SAH cells
Cryopreservation
Storage temperature: -70°C
Effect on Host
Yes, in vitro effects: Cytopathic effects (degeneration, rounding, and clumping of cells)
CPE characterized by rounding of cells, clumping, clustering and degeneration of cells.
Name of Depositor Iowa State Univ. Res. Fndn.
Source
pneumonic pig lungs, United States
References

Paul PS, et al. Highly virulent porcine reproductive and respiratory syndrome viruses which produce lesions in pigs and vaccines that protect pigs against SAID syndrome. US Patent 5,695,766 dated Dec 9 1997

or colder

or colder