I-10 (ATCC® CCL-83)

Organism: Mus musculus, mouse  / 

Organism Mus musculus, mouse
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Gender male
Strain BALB/cJ
Applications
Tested and found negative for ectromelia virus (mousepox).
Clinical Data
male
Antigen Expression
H-2d
Genes Expressed
4-pregnen-3-one(20-hydroxy); progesterone,H-2d
Cellular Products
4-pregnen-3-one(20-hydroxy); progesterone
Virus Resistance
poliovirus 1
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor G Sato
References

Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399

Shin SI. Studies on interstitial cells in tissue culture: steroid biosynthesis in monolayers of mouse testicular interstitial cells. Endocrinology 81: 440-448, 1967. PubMed: 4382264

Basic Documentation
References

Yasamura Y, et al. Establishment of four functional, clonal strains of animal cells in culture. Science 154: 1186-1189, 1966. PubMed: 4288399

Shin SI. Studies on interstitial cells in tissue culture: steroid biosynthesis in monolayers of mouse testicular interstitial cells. Endocrinology 81: 440-448, 1967. PubMed: 4382264