DS-1 (ATCC® CRL-11102)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte  /  Tissue: derived from metastatic site: pleural effusion  /  Disease: lymphangiectasia

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Organism Homo sapiens, human
Tissue derived from metastatic site: pleural effusion
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease lymphangiectasia
Storage Conditions liquid nitrogen vapor phase
Derivation
The DS-1 cell line was established from cells in the pleural effusion from an immunodeficient patient with intestinal lymphangiectasia.


Antigen Expression
HLA Class I, (100%); HLA Class II (38%); plasma cell antigen (PCA) (99%); CD2 (<5%); CD3 (<5%); CD4 -; CD5 -; CD7 -; CD8 -; CD20 -; CD30 -; CD38 -; CD45 -
Receptor Expression
interleukin 6 (IL-6)
Genes Expressed
surface immunoglobulin
Comments
DS-1 is IL-6 dependent.
Complete Growth Medium RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 10 U/ml IL-6, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 cells/mL. Maintain cultures between 1 x 105 and 2 x 106 cells/mL. Recovery of this line from the frozen state is improved by using a feeder layer of irradiated MRC-5 cells (ATCC 55-X).
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 9,12
D16S539: 9,10
D5S818: 11,14
D7S820: 8,14
THO1: 6,9.3
TPOX: 9,11
vWA: 18,19
Isotype IgG; kappa light chain
Name of Depositor The United States of America
References

Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Bock GH, et al. Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin. US Patent 5,556,947 dated Sep 17 1996