Recommendations For Infection:
For best results, plate cells 24 to 48 hours in advance. Infect at 80-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 33°C in a humidified, 5% CO2
atmosphere for 2 to 4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC VR-1735
should be grown in serum free media that contains 1.0 µg/mL TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Incubation: 2-4 days