R6.5.D6.E9.B2 [R6-5-D6] (ATCC^® HB-9580^™)

R6.5.D6.E9.B2 [R6-5-D6] ATCC^® HB-9580^™

frozen

For-Profit: $551.00 Non-Profit: $459.17

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Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1
Age	adult
Strain	BALB/c
Applications	The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.
Storage Conditions	liquid nitrogen vapor phase

Images
Derivation	Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)
Comments	Animals were immunized with the EBV transformed lymphoblast cell line, JY. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml. Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml. Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C

Isotype	mouse IgG2a kappa
Population Doubling Time	12 hours

Name of Depositor	Dana Farber Cancer Inst.
References	Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994 Mice were immunized with the EBV-transformed human lymphoblastoid cell line JY. The antibody binds to ICAM-1(CD54), and inhibits the interaction of ICAM-1 with leukocytes via lymphocyte function antigen 1(LFA-1, CD11a/CD18).

Name of Depositor

Dana Farber Cancer Inst.

References

Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994

Mice were immunized with the EBV-transformed human lymphoblastoid cell line JY.

The antibody binds to ICAM-1(CD54), and inhibits the interaction of ICAM-1 with leukocytes via lymphocyte function antigen 1(LFA-1, CD11a/CD18).

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS
Other Documentation	Cell Micrograph
References	Springer TA, et al. Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994 Mice were immunized with the EBV-transformed human lymphoblastoid cell line JY. The antibody binds to ICAM-1(CD54), and inhibits the interaction of ICAM-1 with leukocytes via lymphocyte function antigen 1(LFA-1, CD11a/CD18).