MEG-01 (ATCC® CRL-2021)

Organism: Homo sapiens, human  /  Cell Type: megakaryoblast  /  Tissue: bone marrow  /  Disease: chronic myelogenous leukemia (CML)

Organism Homo sapiens, human
Tissue bone marrow
Cell Type megakaryoblast
Product Format frozen
Morphology lymphoblast
Culture Properties mixed, adherent and suspension
Biosafety Level 1
Disease chronic myelogenous leukemia (CML)
Age 55 years
Gender male
Applications This cell line is a suitable transfection host.
Karyotype hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present
Derivation
The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.
Clinical Data
male
55 years
Antigen Expression
CD41 +; CD61 +; CDw14 +
Comments
The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.
They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.
The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, platelet).
They are negative for other lymphoid and myeloid seris antigens.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 105 cells/mL into fresh medium in a new flasks. Keep culture below approximate density of 106 cells/mL.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 8
D16S539: 9
D5S818: 13
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16
Population Doubling Time 36 to 48 hrs
Name of Depositor M Tekeuchi, MJ Fitzgerald
Year of Origin 1983
References

Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511

Basic Documentation
References

Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511