p305 (ATCC® 37612)

Applications: shuttle vectorvector permitting construction of fusion proteins  /  Depositors: JC Lang

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Designations p305
Depositors JC Lang
Biosafety Level 1
Vector Information
Size (kb): 4.5999999046325680
Vector: p305 (plasmid)
Promoters: Promoter lac
Construction: pUC18, aph, herpes simplex virus TK
Marker(s):G418R,ampR
Construct size (kb): 4.599999904632568
Features: insert detection: lacZ'
marker(s): ampR, G418R
promoter: lac
replicon: pMB1
Applications
shuttle vector
vector permitting construction of fusion proteins
Comments
SmaI, HincII and EcoRI sites appear in both the polylinker and the aph cassette.
Restriction digests of the clone give the following sizes (kb): HindIII--4.65; HincII--4.65; PstI--3.0, 1.7.
An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of herpes simplex virus tk promoter.
A PvuII (position 628) of pUC18 was changed to BglII. The aph (aminoglycoside phosphotransferase) gene under the control of herpes simplex virus tk promoter was inserted at the BglII site. Aph is transcribed in the same direction as lacZ.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Lang JC, et al. pUC vectors capable of conferring neomycin resistance of eukaryotic cells. Nucleic Acids Res. 16: 7736, 1988. PubMed: 2842735

Dinakar Desai, personal communication

Shipped freeze-dried
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