MDA PCa 2b (ATCC® CRL-2422)

Organism: Homo sapiens, human  /  Tissue: prostate; derived from metastatic site: bone  /  Disease: adenocarcinoma

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue prostate; derived from metastatic site: bone
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 63 years old adult
Gender male
Ethnicity Black
Applications
Cells from this cell line produce tumors in nude mice when injected either subcutaneously or orthotopically (intraprostatic).
This cell line is suitable for studying cell growth regulation by androgens.
Storage Conditions liquid nitrogen vapor phase
Derivation
MDA PCa 2b was established from a bone metastasis of 63 year old Black male with androgen-independent adenocarcinoma of the prostate.
Clinical Data
male
­
63 years old adult
Black
Antigen Expression
prostate specific antigen (PSA)
Receptor Expression
androgen receptor, expressed
Tumorigenic Yes
Effects
Yes, forms tumors in nude mice
Comments
The cell line expresses prostate specific antigen (PSA) and androgen receptor, grows in vitro and in vivo, and is androgen sensitive.
Complete Growth Medium The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 20%
  • 25 ng/ml cholera toxin
  • 10 ng/ml mouse Epidermal Growth Factor
  • 0.005 mM phosphoethanolamine
  • 100 pg/ml hydrocortisone
  • 45 nM selenious acid
  • 0.005 mg/ml bovine insulin
Do not filter complete medium.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  6. Discard supernatant and resuspend cells in fresh growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.
  7. Incubate cultures at 37°C

Subculture Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 10,11
D5S818: 12,13
D7S820: 8,10
THO1: 8,9
TPOX: 8,11
vWA: 16
Name of Depositor NM Navone
References

Navone NM, et al. Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clin. Cancer Res. 3: 2493-2500, 1997. PubMed: 9815652

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQ's
  1. Medium preparation for CRL-2422


    Date Updated: 2/21/2014

References

Navone NM, et al. Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clin. Cancer Res. 3: 2493-2500, 1997. PubMed: 9815652