Human adenovirus 11 (ATCC® VR-12)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus B  /  Product Format: frozen 1.0 mL per vial

Classification Adenoviridae, Mastadenovirus, Human adenovirus B
Deposited As Adenovirus type 11
Agent Human adenovirus 11
Strain Slobitski
Applications
Respiratory research
Biosafety Level 2
Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images ATCC VR-12 micrograph of host effects
Comments
Agglutinates rhesus RBC.  Weakly oncogenic.  Contains soluble group-specific CF antigen.  Efforts at ATCC® to detect polio by antibody-antigen reaction in VR-12™ have yielded negative results.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
KB cells (ATCC® CCL-17™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, cell rounding and sloughing
Name of Depositor M Schaeffer, DA Stevens
Special Collection ATCC
Source Feces, human, Massachusetts, United States
References

Rowe WP, Hartley JW, Huebner RJ. Additional serotypes of the APC virus group. Proc. Soc. Exp. Biol. Med. 91: 260-262, 1956. PubMed: 13297771

Kibrick S, Melendez L, Enders JF. Clinical associations of enteric viruses with particular reference to agents exhibiting properties of the ECHO group. Ann. NY Acad. Sci. 67: 311-325, 1957. PubMed: 13411969

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Feces from patient with paralytic poliomyelitis, Massachusetts. [Type 1 poliovirus also isolated.]

Cross References

Nucleotide (GenBank) : U52569 Human adenovirus type 11 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.