L368 (ATCC® HB-149)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin)
Cellular Products
immunoglobulin; monoclonal antibody; against human beta-2-microglobulin (beta 2 microglobulin)
Comments
Animals were immunized with the RPMI 8866 human B cell line.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not cross-react with mouse, rat, cat or monkey beta-2-microglobulin.
The antibody is not cytotoxic.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor LA Lampson
References

Lampson LA, et al. Striking paucity of HLA-A, B, C and beta2-microglobulin on human neuroblastoma cell lines. J. Immunol. 130: 2471-2478, 1983. PubMed: 6187860

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Lampson LA, et al. Striking paucity of HLA-A, B, C and beta2-microglobulin on human neuroblastoma cell lines. J. Immunol. 130: 2471-2478, 1983. PubMed: 6187860