MYB 2-7.77 (ATCC® CRL-1724)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Permits and Restrictions

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Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) proteins
Cellular Products
immunoglobulin; monoclonal antibody; against myb (v-myb and chicken c-myb) proteins
Comments
Mice were immunized with bp37v.myb protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not block the binding of MYB 2-3.76 (ATCC CRL-1728) or MYB 2-37.63 (ATCC CRL-1726).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1
Name of Depositor J Bishop, GI Evan
References

Evan GI, et al. Isolation of monoclonal antibodies specific for products of avian oncogene myb. Mol. Cell. Biol. 4: 2843-2850, 1984. PubMed: 6084811

Basic Documentation
Restrictions

The depositor has provided these cells for research purposes only and with the following restrictions: (1) no distribution should be made to third parties, (2) any proposed commercial use must be negotiated with the Hooper Foundation, NSW 1542, University of California, San Francisco, California, 94143, (3) all papers reporting any use of these or derived clones should make direct reference to the original publication (Mol. Cell. Biol. 4:2843-2850, 1984).

References

Evan GI, et al. Isolation of monoclonal antibodies specific for products of avian oncogene myb. Mol. Cell. Biol. 4: 2843-2850, 1984. PubMed: 6084811