Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 47085)

Strain Designations: PAO1-LAC  /  Type Strain: no  /  Biosafety Level: 2

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Strain Designations PAO1-LAC
Application
Transformation host
Opportunistic pathogen research
Type Strain no
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Genotype lacIq+ delta(lacZ)M15+ tetA+ tetR+
Genome Sequenced Strain

Pseudomonas aeruginosa PAO1, complete genome

Type Strain no
Comments
For use with the expression vector pUCP19 (ATCC 87110).
Phenotype verified: tetR.
Constructed by stable integration of a mini-D3112 transposable element containing sequences allowing lacZalpha complementation.
Purified genomic DNA from this organism is available as ATCC 47085D-5.
Genome sequenced strain
Medium ATCC® Medium 1631: LB medium (ATCC medium 1065) with 10 mcg/ml tetracycline
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Genotype lacIq+ delta(lacZ)M15+ tetA+ tetR+
Name of Depositor RR Karkhoff-Schweizer
Special Collection NCRR Contract
References

Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342

Stover CK, et al. Complete genome sequence of Pseudomonas aeruginosa PA01, an opportunistic pathogen. Nature 406: 959-964, 2000. PubMed: 10984043

Cross References

Nucleotide (GenBank) : AE004091 Pseudomonas aeruginosa PAO1, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation