Total size of construct (kb): 7.2
Enzyme to excise insert: HindIII
Vector type: plasmid
Vector size (kb): 4.363
Vector ends: HindIII
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.8; PstI--7.2.
A multiple site polymorphism also detected with XmnI and EcoRI, with a common band of 6.0 and alleles of 8.5 and 10 kb.
An unpublished RFLP is detected by this probe when genomic DNA is digested with EcoRI. The published RFLP is revealed with XmnI.
Dryja TP, et al. Chromosome 13 restriction fragment length polymorphisms. Hum. Genet. 65: 320-324, 1984. PubMed: 6319270
Dryja TP, Morton CC. Mapping of seven polymorphic loci on human chromosome 13 by in situ hybridization. Hum. Genet. 71: 192-195, 1985. PubMed: 4065891
Misra BC, Srivatsan ES. Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II. Am. J. Hum. Genet. 45: 565-577, 1989. PubMed: 2577469
Lundberg C, et al. Loss of heterozygosity in human ductal breast tumors indicates a recessive mutation on chromosome 13. Proc. Natl. Acad. Sci. USA 84: 2372-2376, 1987. PubMed: 3031679
Leppert M, et al. A primary genetic map of chromosome 13q. Am. J. Hum. Genet. 39: 425-437, 1986. PubMed: 3464200
Thaddeus P Dryja, personal communication
Freeze-dried E. coli containing the plasmid