Angiogenesis

Angiogenesis is critical to maintaining the circulation of blood to and from the cells of the body. ATCC provides primary cell cultures, cell lines, and assay-ready kits to aid in the study of angiogenesis.


In angiogenesis, new vasculature sprouts from existing blood vessels. This process occurs naturally during growth and healing; it provides newly formed or repaired tissues with a consistent blood flow for the delivery of gasses and nutrients as well as the removal of waste products. Tumors take advantage of this process by secreting vascular growth factors, causing excessive blood vessels to grow into the tumor. By contrast, in coronary artery disease, stroke, and chronic wounds there is an insufficiency of blood vessel growth. Moreover, in diseases such as diabetes, both exaggerated and inadequate angiogenesis may occur in the same patient. To support angiogenesis research, ATCC offers:

  • An assay-ready, high-throughput angiogenesis kit
  • hTERT-immortalized endothelial cells and mesenchymal stem cells
  • Cell matrix gels to support angiogenic tubule formation
  • Primary vascular endothelial and smooth muscle cells

These products support the formation of vascular structures in vitro, which may be used to investigate disease pathogenesis, employed in toxicological screening assays, or for drug development studies.

Angio-Ready™ Assay Ready System

Angio-Ready™ is an assay-ready, single-use kit designed for the high-throughput monitoring of angiogenesis. This kit comprises the ideal ratio of GFP-expressing hTERT-immortalized endothelial cells pre-mixed with hTERT-immortalized mesenchymal stem cells, and is supplied with complete growth medium. Angio-Ready does not require antibodies, and is dyeless, matrixless, and easy to perform in a 96-well plate.



Are you Angio-Ready? Choose from two available sizes:

Two assays
Ten assays

hTERT-immortalized Vascular Cells

ATCC has a multitude of hTERT-immortalized cells, which combine the in vivo nature of primary cells with a cell line's ability to survive continuously. TeloHAEC cells, for example, express a panel of endothelial cell surface proteins, and undergo tubule formation in culture. hTERT-immortalized mesenchymal stem cells (ASC52telo) support the vascular structures, and in angiogenic culture can differentiate into tunica media-like smooth muscle tissue.

 

hTERT-immortalized Aortic Endothelial Cells

hTERT-immortalized Microvascular Endothelial Cells

hTERT-immortalized Mesenchymal Stem Cells

Primary Vascular Cells

ATCC offers an array of vascular primary cells, such as endothelial, smooth muscle, and fibroblast cells. Primary cells represent an excellent source of physiologically relevant controls for toxicological experiments or 3D culture models.


Primary Endothelial Cells

Primary Aortic Smooth Muscle Cells

Primary Coronary Artery Smooth Muscle Cells

Primary Pulmonary Artery Smooth Muscle Cells

Primary Fibroblasts