Pseudomonas aeruginosa (Schroeter) Migula (ATCC® 10145)

Strain Designations: [CCEB 481, MDB strain BU 277, NCIB 8295, NCPPB 1965, NCTC 10332, NRRL B-771, R. Hugh 815]  /  Type Strain: yes  /  Biosafety Level: 2

Strain Designations [CCEB 481, MDB strain BU 277, NCIB 8295, NCPPB 1965, NCTC 10332, NRRL B-771, R. Hugh 815]
Application
Bacterial resistance testing adhesives
Bacterial resistance testing paint
Detection of aerosols
Produces lipases active at pH 5.5 and 7.5
Produces lipases active at pH 9.0 at 60C
Quality control strain
Reference material
Quality control strain for API and Sensititre products
Positive control for molecular detection in bioaerosols
Opportunistic pathogen research
Water testing
Type Strain yes
Biosafety Level 2
Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Intended Use
Preceptrol® yes
Type Strain yes
Medium Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor F Kavanagh
Chain of Custody
ATCC <-- F Kavanagh <-- Merck Sharp & Dohme
References

Opinion 36. Designation of strain ATCC 10145 as the neotype strain of Pseudomonas aeruginosa (Schroeter) Migula. Int. J. Syst. Bacteriol. 20: 15-16, 1970.

J. Gen. Microbiol. 25: 398, 1961.

Int. Bull. Bacteriol. Nomencl. Taxon. 14: 69-84, 1964.

ASTM International Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 2574-06.

ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 4783-01e1.

Lange JL, et al. Application of flow cytometry and fluorescent in situ hybridization for assessment of exposures to airborne bacteria. Appl. Environ. Microbiol. 63: 1557-1563, 1997. PubMed: 9097451

Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980.

Hou CT. pH dependence and thermostability of lipases from cultures from the ARS Culture Collection. J. Ind. Microbiol. 13: 242-248, 1994.

BD Oxi/Ferm II QC Set. BD Becton Dickinson; Oxi/Ferm

Colilert. IDEXX.

Colisure. IDEXX.

Sensititre™ Automated Gram Negative ID. Thermo Scientific™ Sensititre™ Systems, Trek Diagnostic Systems.

Waters--Examination for legionellae including Legionella pneumophila. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 3896:1998.

Water microbiology. Method 4: Coliforms--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.4-1995.

Water microbiology. Method 5: Coliforms--Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.5-1995.

Water microbiology. Method 10: Pseudomonads--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.10-1995.

Water microbiology. Method 11: Pseudomonads -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.11-1995.

Water microbiology. Method 12: Pseudomonas aeruginosa--Estimation of most probable number(MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.12-1995.

Water microbiology. Method 13: Pseudomonas aeruginosa- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.13-1995.

Water microbiology. Method 16: Bactericidal efficiency of water disinfecting tablets. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.16:1999.

Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.18:2001.

Colilert-18 QC Set. IDEXX.

ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM.

Pseudalert QC Set. IDEXX.

Colitag™ Test Kit QC Set. CPI International

Cross References

Nucleotide (GenBank) : K02353 Pseudomonas aeruginosa 5S ribosomal RNA.

Nucleotide (GenBank) : Y00334 Pseudomonas aeruginosa ssr gene for 6S RNA.

Nucleotide (GenBank) : X12644 Pseudomonas aeruginosa ffs gene for 4.5S RNA.

Nucleotide (GenBank) : Y00432 Pseudomonas aeruginosa gene for 23S ribosomal RNA.

Nucleotide (GenBank) : X07317 Pseudomonas aeruginosa gene for blue copper protein azurin.

Nucleotide (GenBank) : AF094713 Pseudomonas aeruginosa strain ATCC 10145 16S ribosomal RNA gene, partial sequence.

Nucleotide (GenBank) : X51319 Pseudomonas aeruginosa DNA for cytochrome c-551 and the C-terminal of nitrite reductase (EC 1.9.3.2).