RPMI 8226 (ATCC® CRM-CCL-155)

Organism: Homo sapiens, human  /  Cell Type: B lymphocyte (KRAS CRM)  /  Tissue: peripheral blood  /  Disease: plasmacytoma; myeloma

Organism Homo sapiens, human
Tissue
peripheral blood
Cell Type B lymphocyte (KRAS CRM)
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease plasmacytoma; myeloma
Age 61 years
Gender male
Applications For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. ISO Guide 34:2009

This cell line is a suitable transfection host.

Intended Use


Certified Reference Material produced under an ISO Guide 34:2009 accredited process. 


    ACLASS Accredited Reference Material Producer AR-1384

Storage Conditions liquid nitrogen vapor phase
Karyotype Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres.
Clinical Data
male
Antigen Expression
HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
Genes Expressed
immunoglobulin light chain,HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
Cellular Products
immunoglobulin light chain
Virus Susceptibility Human poliovirus 1
Herpes simplex virus
Vaccinia virus
Vesicular stomatitis virus
Comments Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.
There is no evidence of heavy chain production (cytoplasmic or secreted).

Certified Reference Material produced under an ISO Guide 34:2009 accredited process.

Item has been tested for KRAS mutation (p.G12A c.35G>C)

Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 105 viable cells/mL. Do not allow the cell density to exceed 2 to 3 X 106 cells/mL.
Interval: Maintain cell density between 5 X 105 and 2 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Culture Conditions
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18
Isoenzymes
G6PD, A
Isotype lambda light chain
Year of Origin June, 1966
References

Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436

Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

Basic Documentation
FAQ's
  1. Agreement to transfer to a CRO


    Date Updated: 1/2/2013

References

Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436

Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066

Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375