Human adenovirus 5 (ATCC® VR-5)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus C  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Adenoviridae, Mastadenovirus, Human adenovirus C
Deposited As Adenovirus type 5
Agent Human adenovirus 5
Strain Adenoid 75
Applications
Viruscide testing
Respiratory research
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Not inactivated by ether. Contains soluble group-specific complement fixation antigen. Agglutinates rat red blood cells incompletely.
Effect on Host
CPE, refractile rounding, clumping and sloughing
Recommended Host
HeLa (ATCC CCL-2)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days
Effect on Host
CPE, refractile rounding, clumping and sloughing
Name of Depositor RJ Huebner, National Institutes of Health; M Shaffer, Public Health Research Institute of the City of New York
Special Collection NCRR Contract
Source Spontaneously degenerating tissue culture of adenoid tissue from a 4-year-old girl with enlarged chronically infected tonsils, Washington DC, 1953
References

Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006.

Chemical disinfectants and antiseptics --- Virucidal quantitative suspension test for chemical disinfectants and antiseptics used in human medicine --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 14476:2005.

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Huebner RJ, et al. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc. Soc. Exp. Biol. Med. 84: 570-573, 1953. PubMed: 13134217

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation